No, you can use all available relays for posting, until the relay operator decides to blacklist the bot pubkey.
The notes from a bot look like a note from a normal user.
Which nostr library you will use for your bot?
npub1nq…kkz86
2023-01-21 14:47:00
in reply to nevent1q…ulel
Author Public Key
npub1nqu6xm23khceqjtlnvkjdrhq3yp7a3l4a5hf4c7wadwm3x4thggqjkkz86Published at
2023-01-21 14:47:00Event JSON
{
"id": "ea32ed03571613a5f45f94bc6dabc263af2fd8c7f22b1d9fa23318220d42ec8d",
"pubkey": "9839a36d51b5f190497f9b2d268ee08903eec7f5ed2e9ae3ceeb5db89aabba10",
"created_at": 1674312420,
"kind": 1,
"tags": [
[
"e",
"8760c16e1b7add8efdab28a8ed10d4a71d9e0db8ecfee7dd9f811abb704120b8",
"",
"reply"
],
[
"p",
"ddfbb06a722e51933cd37e4ecdb30b1864f262f9bb5bd6c2d95cbeefc728f096"
]
],
"content": "No, you can use all available relays for posting, until the relay operator decides to blacklist the bot pubkey.\n\nThe notes from a bot look like a note from a normal user. \n\nWhich nostr library you will use for your bot?",
"sig": "4d523520591233e3a26a24d537cc976f14d88471f188351c7937e279fe6ed38180f3915806df3ee249456cf3c3661af03378296337aa7c938ebd1710c5192e34"
}